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1.
A contraction of: it was not
'Twasn't the night before christmas.
von The-Whale-Bullier 17. April 2011
 
2.
the area on a man between the nutsack and the asshole
if twasnt there - your insides would fall out
von Beaver660 5. August 2006
 
3.
The Old English new age combination of the words: It, Was, and Not.
"What night did that burglar break into the house?"

"T'wasn't the night before Christmas."

"You're a douche."
von Benjiforce1 30. September 2010
 
4.
Its an alternate form of saying the word "Taint."
She tickled the area near my undercarriage that twasnt my balls or ass.
von Steve 13. Dezember 2003